Skip to contents

Visualize multi-orientation environmental-species correlation heatmaps (adaptive sizing)

Source: R/gglink_heatmaps_2.R
gglink_heatmaps_2.Rd

An improved version of gglink_heatmaps that sizes each heatmap quadrant independently according to its own number of variables. All tiles share the same size; larger env blocks simply extend further. The central species network is always kept at the centre of the canvas.

Usage

gglink_heatmaps_2(
  env,
  spec,
  env_select = NULL,
  spec_select = NULL,
  spec_layout = "circle_outline",
  spec_orientation = c("up", "down", "left", "right"),
  spec_relation = TRUE,
  relation_method = c("correlation", "mantel"),
  cor.method = c("pearson", "kendall", "spearman"),
  cor.use = c("everything", "all", "complete", "pairwise", "na"),
  mantel.method = c("mantel", "mantel.partial", "mantelhaen.test", "mantel.correlog"),
  mantel.method2 = c("pearson", "kendall", "spearman"),
  mantel.alternative = c("two.sided", "less", "greater"),
  drop_nonsig = FALSE,
  comparisons = TRUE,
  comparisons_groups = NULL,
  shape = 22,
  distance = 3,
  HeatmapLabelSize = 5,
  HeatmapSigSize = 5,
  HeatmapColorBar = NULL,
  HeatmapLabelOrient = 0,
  SigLineWidth = c(0.5, 2),
  SigLineColor = c("#fdbb84", "#d7301f"),
  HeatmapPointSize = 5,
  CorePointSize = 8.5,
  HeatmapPointFill = "#de77ae",
  CorePointFill = "#41b6c4",
  HeatmapTileColor = NA,
  HeatmapTileSize = 0,
  HeatmapScale = 1,
  SigLineAlpha = 0.5,
  fontsize = 5,
  orientation = c("top_right", "bottom_right", "top_left", "bottom_left"),
  r = 6,
  group_layout = c("circle", "row", "column", "square", "diamond", "triangle",
    "triangle_down", "snake"),
  anchor_dist = 6,
  scale_networks = TRUE,
  nrow = NULL,
  ncol = NULL
)

Arguments

env

Data Frame A data frame or matrix containing environmental variables. Each column represents an environmental factor.

spec

Data Frame A data frame or matrix containing species abundance or trait data. Each column represents a species or taxonomic unit.

env_select

Optional list specifying the column indices (or names) of environmental variables to include in each environmental block. Each list element corresponds to one quadrant of the heatmap layout.

spec_select

Optional list specifying column indices (or names) of species to include. If multiple elements are provided, they define separate species clusters in the visualization.

spec_layout

Character or character vector (default = "circle_outline"). Spatial arrangement of species nodes for each central network. If a single value, applied to all spec blocks. If a vector, length must match the number of spec blocks; each element specifies the layout for that block in order. Valid options: "circle_outline", "diamond_outline", "rectangle_outline", "square_outline".

spec_orientation

Character spec_oritation. Options include: "up","down","left","right"

spec_relation

Logical (defalt = TRUE) Whether to compulate the ralationship of spec

relation_method

Character Method for computing relationships between species and environmental factors. Options are `"correlation"` or `"mantel"`.

cor.method

Character Correlation method to use when `relation_method = "correlation"`. One of `"pearson"`, `"kendall"`, or `"spearman"`.

cor.use

Character Method for handling missing values in correlation computation. One of `"everything"`, `"all"`, `"complete"`, `"pairwise"`, or `"na"`.

mantel.method

Character Type of Mantel test to use when `relation_method = "mantel"`. Options include `"mantel"`, `"mantel.partial"`, `"mantelhaen.test"`, and `"mantel.correlog"`.

mantel.method2

Character Correlation coefficient used in the Mantel test. One of `"pearson"`, `"kendall"`, or `"spearman"`.

mantel.alternative

Character Alternative hypothesis for Mantel test. One of `"two.sided"`, `"less"`, or `"greater"`.

drop_nonsig

Logical if `TRUE`, non-significant correlations are dropped from the final visualization.

comparisons

Logical (default = TRUE). Whether to perform species–environment correlation or Mantel analysis. If FALSE, no spec–env links are computed or drawn.

comparisons_groups

List or NULL (default = NULL). When comparisons = TRUE, constrains which (env_block, spec_block) pairs are analyzed. Each element must be a length-2 character vector: c(env_block_name, spec_block_name), e.g. list(c("Env01", "Spec01"), c("Env02", "Spec01")). Block names must match names(env_select) and names(spec_select). If NULL, all env–spec block pairs are analyzed (default).

shape

Intrger Integer or numeric specifying the shape of species nodes in the plot (passed to `geom_point()`).

distance

Numeric the offset distance between central nodes and the environmental heatmaps.

HeatmapLabelSize

Numeric (default = 5) Text size for heatmap axis labels (ID/Type).

HeatmapSigSize

Numeric (default = 5) Text size for significance symbols (e.g. `*`, `**`, `***`) inside heatmap tiles.

HeatmapColorBar

NULL or list Controls the colorbar palettes used by each heatmap quadrant.

- If `NULL`, the built-in default palettes are used (same as current behavior). - If a list of length 2 with names `low` and `high`, each should be a character vector of colors (recycled if shorter) used across quadrants. - If a list of length equal to the number of quadrants, each element should be either `c(low, high)` or `list(low=..., high=...)` for that quadrant (in order). - example `HeatmapColorBar = list( c("#2166ac", "#b2182b"), # 第1个象限 low/high c("#1b7837", "#762a83"), # 第2个 c("#4393c3", "#d6604d"), # 第3个 c("#92c5de", "#f4a582") # 第4个 )`

HeatmapLabelOrient

Numeric (default = 0) Rotation angle (in degrees) for heatmap axis labels (ID/Type). Use this to avoid overlap of the top/bottom labels; e.g. 45 or 90.

SigLineWidth

Numeric vector of length 2 (default = c(0.5, 2)) Controls the minimum and maximum line width for species–environment links, scaled by significance (p-value). Smaller p leads to thicker lines.

SigLineColor

Character vector (length 2, default = c("#fdbb84", "#d7301f")) Colors used for the species–environment link color gradient, corresponding to low and high correlation values respectively.

HeatmapPointSize

Numeric (default = 5) Point size for the heatmap diagonal nodes.

CorePointSize

Numeric (default = 8.5) Point size for the central species nodes.

HeatmapPointFill

Character (default = "#de77ae") Fill color for heatmap diagonal points.

CorePointFill

Character (default = "#41b6c4") Fill color for central species nodes.

HeatmapTileColor

Character or NA (default = NA) Border color for heatmap tiles (passed to `geom_tile(colour=...)`).

HeatmapTileSize

Numeric (default = 0) Border line width for heatmap tiles (passed to `geom_tile(size=...)`).

HeatmapScale

Numeric (default = 1) Global scale factor for the overall heatmap size. Values > 1 enlarge the whole heatmap layout, while values < 1 shrink it.

SigLineAlpha

Numeric (default = 0.5) Transparency for species-network to heatmap link lines. Must be between 0 and 1.

fontsize

Numeric (default = 5) (Deprecated) Use `HeatmapLabelSize` instead.

orientation

Character Character vector defining which heatmap quadrants to display. Can include any combination of `"top_right"`, `"bottom_right"`, `"top_left"`, and `"bottom_left"`.

r

Numeric radius of the central species layout (in plot units).

group_layout

Character (default = "circle"). Arrangement of multiple species networks when spec_select has multiple elements. Options: "circle", "row", "column", "square", "diamond", "triangle", "triangle_down", "snake".

anchor_dist

Numeric (default = 6). Distance between species networks when multiple spec_select blocks are used.

scale_networks

Logical (default = TRUE). If TRUE, normalize each species network to the same scale (radius r) before placing on anchors. If FALSE, r is the minimum network radius; larger networks scale proportionally by node count.

nrow

Integer (default = NULL). Number of rows for group_layout = "row", "column", or "snake".

ncol

Integer (default = NULL). Number of columns for group_layout = "row", "column", or "snake".

Value

A list of length 3: - [[1]]: ggplot object with straight link segments. - [[2]]: ggplot object with curved link segments. - [[3]]: data.frame of full species-environment correlation statistics (unfiltered, not affected by drop_nonsig), with columns ID, Type, Correlation, Pvalue, spec_block, env_block, and method (e.g. "correlation" or "mantel").

Examples

NULL
#> NULL